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Small scale fermentation system - Ambr® 250 High Throughput

Ambr® 250 High Throughput is a fully automated bioreactor system for small scale fermentation or cell culture using 100-250mL single-use bioreactors controlled by an automated liquid handling platform.

The system provides a high precision environment that supports the demands of microbial fermentation and mammalian cultures.

Ambr® 250 High Throughput provides a rapid and reliable scale-down model to explore a wide range of conditions with excellent scalability to larger bioreactors.

Ambr® 250 High Throughput workstation:

Ambr® 250 High Throughput workstation offers independent, parallel control of 12 or 24 single-use bioreactors (100-250ml working volume).

Fully automated system - media fill, inoculation, sampling and feeding
Individual control for each bioreactor vessel - temperature, impeller speed, pH and DO
Integrated control of multiple bioreactors (12 or 24)

Small scale fermentation system - view a video demonstration of the key Ambr® 250 High Throughput processes

Ambr® 250 High Throughput vessels

Single use 100-250ml bioreactor vessels have been developed for use with the system - avoiding the need to clean reactors and sensors between runs.

The design includes a novel "easy connect" process enabling all gas, liquid and sensor connections to be made, quickly and simply, for fast turnaround between experiments.

Integrated cell culture analyzers

A range of integrated cell culture analysers are available for the Ambr® 250 High Throughput system.

Integrated cell culture analysers - view further information

small scale fermentation -Ambr 250 High Throughput vessels

Ambr® 250 Microcarrier Vessel

The new vessel type has been developed to support microcarrier and other cultures requiring specialized mixing capabilities.

The vessel is unbaffled and has a single 'elephant ear' shaped impeller which is larger than the standard 'pitched blade' impeller. The configuration minimizes shear stress. and performs a radial mix of cultures at lower stirring speeds while maintaining cell functionality and other quality aspects. The vessel is suitable for:

Process development and process optimization
Microcarrier-based mammalian cell culture applications
Other cultures requiring specialized mixing at slower impeller speed

Online biomass option for microbial applications

This technology integration providesfurther step forward in advanced microbial process monitoring and control:

Real-time continuous growth monitoring for microbial applications
Fully automated and non-invasive analysis; does not interrupt experiment or reduce culture levels
Effective bubble discrimination in high aeration and agitation environments
Reduces the burden of frequent offline manual sampling; freeing up valuable operator time
Feedback control of process events possible based on real-time biomass data

Click to view a PDF of the schematic

Ambr® 250 High Throughput offers:

A better tool for process development of microbes
Rapid evaluation of a wide range of conditions and cell line/strain performance
Excellent scalability to large-scale, bench top bioreactors

Applications include:

Small scale fermentation system
Process development for biopharma and industrial biotech
Process robustness experimentation in support of QbD studies
Automation of DOE approaches to process automation