Small scale fermentation system - Ambr 250 High Throughput ambr 250 high throughput

Small scale fermentation system - Ambr® 250 High Throughput

Ambr® 250 High Throughput is a fully automated bioreactor system for small scale fermentation or cell culture using 100-250mL single-use bioreactors controlled by an automated liquid handling platform.

The system provides a high precision environment that supports the demands of microbial fermentation and mammalian cultures.

Ambr® 250 High Throughput provides a rapid and reliable scale-down model to explore a wide range of conditions with excellent scalability to larger bioreactors.

 

Small scale fermentation system
Ambr® 250 High Throughput workstation:

Ambr® 250 High Throughput workstation offers independent, parallel control of 12 or 24 single-use bioreactors (100-250ml working volume).

  • Fully automated system - media fill, inoculation, sampling and feeding
  • Individual control for each bioreactor vessel - temperature, impeller speed, pH and DO
  • Integrated control of multiple bioreactors (12 or 24)

 

 

Ambr® 250 High Throughput vessels

Single use 100-250ml bioreactor vessels have been developed for use with the system - avoiding the need to clean reactors and sensors between runs.

The design includes a novel "easy connect" process enabling all gas, liquid and sensor connections to be made, quickly and simply, for fast turnaround between experiments.

Integrated cell culture analyzers

A range of integrated cell culture analysers are available for the Ambr® 250 High Throughput system.

 

 

small scale fermentation -Ambr 250 High Throughput vessels
microcarrier vessels

 

 

Ambr® 250 Microcarrier Vessel

The new vessel type has been developed to support microcarrier and other cultures requiring specialized mixing capabilities.

The vessel is unbaffled and has a single 'elephant ear' shaped impeller which is larger than the standard 'pitched blade' impeller. The configuration minimizes shear stress. and performs a radial mix of cultures at lower stirring speeds while maintaining cell functionality and other quality aspects. The vessel is suitable for:

  • Process development and process optimization
  • Microcarrier-based mammalian cell culture applications
  • Other cultures requiring specialized mixing at slower impeller speed

 

 

Ambr 250 High Throughput biomass

 

Online biomass option for microbial applications

This technology integration providesfurther step forward in advanced microbial process monitoring and control:

  • Real-time continuous growth monitoring for microbial applications
  • Fully automated and non-invasive analysis; does not interrupt experiment or reduce culture levels
  • Effective bubble discrimination in high aeration and agitation environments
  • Reduces the burden of frequent offline manual sampling; freeing up valuable operator time
  • Feedback control of process events possible based on real-time biomass data

 

 

Ambr® 250 High Throughput offers:
  • A better tool for process development of microbes
  • Rapid evaluation of a wide range of conditions and cell line/strain performance
  • Excellent scalability to large-scale, bench top bioreactors

 

Applications include:
  • Small scale fermentation system
  • Process development for biopharma and industrial biotech
  • Process robustness experimentation in support of QbD studies
  • Automation of DOE approaches to process automation