Fully Automated Bioreactor System for Fermentation or Cell Culture

Ambr® 250 High Throughput workstation offers independent, parallel control of 12 or 24 single-use bioreactors (100-250ml working volume).

• Fully automated system - media fill, inoculation, sampling and feeding
• Individual control for each bioreactor vessel - temperature, impeller speed, pH and DO
• Integrated control of multiple bioreactors (12 or 24)

View a video demonstration of the key Ambr® 250 High Throughput processes

Single use 100-250ml bioreactor vessels have been developed for use with the system - avoiding the need to clean reactors and sensors between runs.

The design includes a novel "easy connect" process enabling all gas, liquid and sensor connections to be made, quickly and simply, for fast turnaround between experiments.

A range of integrated cell culture analysers are available for the Ambr® 250 High Throughput system.

Integrated cell culture analysers - view further information

The new vessel type has been developed to support microcarrier and other cultures requiring specialized mixing capabilities.

The vessel is unbaffled and has a single 'elephant ear' shaped impeller which is larger than the standard 'pitched blade' impeller. The configuration minimizes shear stress. and performs a radial mix of cultures at lower stirring speeds while maintaining cell functionality and other quality aspects. The vessel is suitable for:

• Process development and process optimization
• Microcarrier-based mammalian cell culture applications
• Other cultures requiring specialized mixing at slower impeller speed

This technology integration providesfurther step forward in advanced microbial process monitoring and control:

• Real-time continuous growth monitoring for microbial applications
• Fully automated and non-invasive analysis; does not interrupt experiment or reduce culture levels
• Effective bubble discrimination in high aeration and agitation environments
• Reduces the burden of frequent offline manual sampling; freeing up valuable operator time
• Feedback control of process events possible based on real-time biomass data

Click to view a PDF of the schematic